• Youngjun Song, Sejung Kim, Michael J. Heller and Xiaohua Huang, “DNA multi‑bit non‑volatile memory and bit‑shifting operations using addressable electrode arrays and electric field‑induced hybridization”, Nature Communications, 18;9(1):281 (2018). doi: 10.1038/s41467-017-02705-8 (PDF).
  • Wei-Keung Chu, Peter Edge, Hosuk Lee, Vikas Bansal, Vineet Bafna, Xiaohua Huang and Kun Zhang, “Ultraaccurate genome sequencing and haplotyping of single human cells”. PNAS, USA, 114 (47) 12512-12517 (2017). doi: 10.1073/pnas.1707609114 (PDF).
  • Matthew Walsh and Xiaohua Huang, “Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy”. Nucleic Acids Research, 1;45(21):e175 (2017). doi: 10.1093/nar/gkx833 (PDF).
  • Matthew T. Walsh, Alexander P. Hsiao, Ho Suk Lee, Zhixia Liu and Xiaohua Huang, “Capture and enumeration of mRNA transcripts from single cells using a microfluidic device”, Lab on a Chip, 15(14):2968-80 (2015). doi: 10.1039/c5lc00445d (PDF).
  • Matthew T. Walsh, Eric E Roller, Kwang-su Ko and Xiaohua Huang, “Measurement of DNA polymerase incorporation kinetics of dye-labeled nucleotides using total internal reflection fluorescence microscopy”. Biochemistry, 54(26):4019-21 (2015). doi:10.1021/acs.biochem.5b00269.
  • Ho Suk Lee, Wai Keung (Eric) Chu, Kun Zhang and Xiaohua Huang, “Microfluidic devices with permeable polymer barriers for capture and transport of biomolecules and cells”. Lab on a Chip, 13:3389-3397 (2013), doi: 10.1039/C3LC50280E (PDF).
  • Nora Theilacker, Eric E. Roller, Kristopher D. Barbee, Matthias Franzreb and Xiaohua Huang, “Multiplexed protein analysis using encoded antibody-conjugated microbeads”, Journal of Royal Society Interface, 8(61):1104-13 (2011), doi: 10.1098/rsif.2010.0594 (PDF).
  • Kristopher D. Barbee, Matt Chandrangsu and Xiaohua Huang, “Fabrication of DNA polymer brush arrays by destructive micropatterning and rolling-circle amplification”. Macromolecular Bioscience, 11(5):607-17 (2011), doi: 10.1002/mabi.201000373 (PDF).
  • Aric Joneja and Xiaohua Huang, “Linear nicking endonuclease-mediated strand displacement DNA amplification”, Analytical Biochemistry, 414(1):58-69 (2011), doi: 10.1016/j.ab.2011.02.025 (PDF).
  • Kristopher D. Barbee, Alexander Hsiao, Eric E Roller and Xiaohua Huang, “Multiplexed protein detection using antibody-conjugated microbead arrays in a microfabricated electrophoretic device”, Lab on a Chip, 10(22), 3084-3093 (2010). doi: 10.1039/c0lc00044b (PDF).
  • Alexander P. Hsiao, Kristopher D. Barbee and Xiaohua Huang, “Microfluidic device for capture and isolation of single cells”. Proceedings of SPIE, 7759 77590W-1, doi: 10.1117/12.861563 (2010) (PDF).
  • Ying-Ja Chen, Eric Roller and Xiaohua Huang, “DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device”. Lab Chip, 10(9):1153-9 (2010), doi:10.1039/B921417H (PDF)
  • Ying-Ja Chen and Xiaohua Huang, “DNA Sequencing By Denaturation: Principle and Thermodynamic Simulations”, Analytical Biochemistry, 384(1), 170-179 (2009).
  • Carl Fuller, Lyle Middendorf, Steven A. Benner, George M. Church, Timothy Harris, Xiaohua Huang, Steven B. Jovanovich, John R. Nelson, Jeffrey A. Schloss, David C. Schwartz, and Dimitri V. Vezenov, “The challenges of sequencing by synthesis”, Nature Biotechnology, 27(11), 1013-1023 (2009), doi:doi:10.1038/nbt.1585
  • Aric Joneja and Xiaohua Huang, “A Device for Automated Hydrodynamics Shearing of Genomic DNA”. BioTechniques, 46(7), 553-556 (2009)
  • Daniel Branton, et al, “The potential and challenges of nanopore sequencing”, Nature Biotechnology, 26(10), 1146-1153 (2008), doi:10.1038/nbt.1495 (PDF).
  • Kristopher D. Barbee and Xiaohua Huang, “Magnetic assembly of high-density DNA arrays for genomic analyses”, Analytical Chemistry, 80(6), 2149-2154 (2008), doi: 10.1021/ac702192y (PDF).
  • Xiaohua Huang, “Genomes, Genome Technologies and Medicine”, in An Introductory Text to Bioengineering, Shu Chien, Peter C. Y. Chen and Y.C. Fung, Eds.; World Scientific Publishing Co. Pte. Ltd, Singapore, 2008; Chapter 27, pp 473-485.
  • Martha Bulyk, Xiaohua Huang, Yen Choo and George M. Church, “Exploring the DNA binding specificities of zinc fingers with DNA microarrays”, PNAS, USA, 98(13), 7158-7163 (2001) (PDF).
  • Xiaobo Zhong, Paul M. Lizardi, Xiaohua Huang, Patricia L. Bray-Ward and David C. Ward, “Visualization of Oligonucleotide Probes and Point Mutations in Interphase Nuclei and DNA Fibers Using Rolling Circle DNA Amplification”, Proceedings of the National Academy of Sciences, U.S.A., 98(7), 3940-3945 (2001).
  • Tulin Ozcan, Nicole Burki, Vinita Parkash, Xiaohua Huang, Tanya Pejovic, Maurice J. Mahoney and David C. Ward, “Cytogenetical diagnosis in paraffin-embedded fetoplacental tissue using comparative genomic hybridization”, Prenatal Diagnosis, 20(1), 41-44 (2000) (PDF).
  • Paul M. Lizardi, Xiaohua Huang, Zhengrong Zhu, Patricia Bray-Ward, David C. Thomas and David C. Ward, “Mutation detection and single molecule counting using rolling circle amplification”, Nature Genetics, 19, 225-232 (1998), doi:10.1038/898 (PDF).
  • Xiaohua Huang, “Probing Ligand Binding Dynamics and Folding of Myoglobin Using a Random Library of Myoglobin Mutants with Single Amino Acid Mutations”, Ph.D. thesis, Stanford University, Stanford, California 94305, USA(1997).
  • Xiaohua Huang and Steven G. Boxer, “Discovery of new ligand binding pathways in myoglobin by random mutagenesis”, Nature Structural Biology, 1(4), 226-229 (1994) (PDF).

Patents and Inventions

Patents - Issued

  • Xiaohua Huang, “Methods and systems for direct real-time sequencing of single DNA molecules”, United States Patent No. US 9,382,584 B2 (PDF) (Issued July 5, 2016).
  • Xiaohua Huang and Kristopher D Barbee, “Methods and devices for biomolecular arrays”, United States Patent No. US 9063133B2 (PDF) (Issued June 23, 2015).
  • Xiaohua Huang and Eric E Roller, “Mostly natural DNA sequencing by synthesis”, United States Patent No. US 8772473 B2 (PDF) (Issued July 8, 2014).
  • Xiaohua Huang and Paul M. Lizardi, “Single molecule analysis and target-mediated ligation of bipartite primers”, United States Patent No. US 6316229 B1 (PFD) (Issued November 13, 2001).

Patents - Pending

  • Xiaohua Huang, “Duplicating DNA with contiguity barcodes for genome and epigenome sequencing”, US application No. US20160265039A1, WIPO International Publication No. WO 2015/073693 A1 (May, 2015)
  • Kun Zhang and Xiaohua Huang, “Accurate genome sequencing of single cells by single-stranded amplification and sequencing”, Patent Application. No. PCT/US2013/059086. WIPO International Publication No. WO 2014043140 A1 (2014).
  • Xiaohua Huang, “Hybrid nanopore devices and methods for single-molecule protein sequencing”, Patent Application (2019).

Invention disclosures

  • Xiaohua Huang, “Methods of DNA multiplexed Encoding”, UCSD invention disclosure (2013).