Publications

Theilacker, N., Roller, E., Barbee, K.D., Franzreb, M., Huang, X. Multiplexed Protein Analysis Using Encoded Antibody-conjugated Microbeads. J. R. Soc. Interface. (2011) DOI:10.1098/rsif.2010.0594 (In Press) (pdf)

Barbee, K.D., Chandrangsu, M., Huang, X. Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling Circle Amplification. Macromol. Biosci. (2011) DOI: 10.1002/mabi.201000373 (In Press) (pdf)

Barbee, K.D.; Hsiao, A.P.; Roller, E.E.; Huang, X. Multiplexed protein detection using antibody-conjugated microbead arrays in a microfabricated electrophoretic device. Lab on a chip (2010) DOI: 10.1039/C0LC00044B (In Press) (pdf)

Joneja, A.; Huang, X. Linear nicking endonuclease-mediated strand displacement DNA amplification. Accepted Analytical Biochemistry

Chen, Y.-J.; Roller, E.E.; Huang, X. DNA Sequencing by Denaturation: Experimental proof of concept with an integrated fluidic device. Lab on a Chip (2010) May 7;10(9):1153-9. Epub 2010 Feb 9. (ABSTRACT) (pdf)

Barbee, K.D.; Hsiao, A.P.; Heller, M.J.; Huang, X. Electric Field Directed Assembly of High-Density Microbead Arrays. Lab on a Chip (2009) Nov 21; 9 (22) 3268-3274 (ABSTRACT) (pdf)

Fuller, C.W., Middendorf, L.R., Benner S.A., Church, G.M., Harris, T., Huang, X., Jovanovich, S.B., Nelson, J.R., Schloss, J.A., Schwartz, D.C., Vezenov, D.V. The Challenges of Sequencing by Synthesis. Nature Biotechnology (2009) Nov; 27 (11) 1013-1023 (ABSTRACT) (pdf)

Joneja, A.; Huang, X. A Device for Automated Hydrodynamic Shearing of Genomic DNA. BioTechniques (2009) Jun; 46 (7) 553-556. (ABSTRACT) (pdf)

Chen, Y.-J.; Huang, X. DNA Sequencing by Denaturation: Principles and Thermodynamic Simulations. Analytical Biochemistry (2009) Jan 1; 384 (1) 170-179. (ABSTRACT) (pdf)

Branton, D. et al. The Potential and Challenges of Nanopore Sequencing. Nature Biotechnology (2008) Oct 9; 26 (10) 1146-53. (ABSTRACT) (pdf)

Huang, X. (2008) Genomes, Genomic Technologies and Medicine. In Chien, S.; Chen, P.C.Y.; Fung, Y.C. (Eds.) An Introductory Text to Bioengineering (Advanced Series in Biomechanics) (2nd ed.). Hackensack: World Scientific Publishing.

Barbee, K.D.; Huang, X. Magnetic Assembly of High-Density DNA Arrays for Genomics Analyses. Analytical Chemistry (2008) Mar 15; 80 (6) 2149-54. (ABSTRACT) (pdf)

Bulyk, M.L.; Huang, X.; Choo, Y.; Church, G.M.  Exploring the DNA-Binding Specificities of Zinc Fingers with DNA Microarrays. Proc. Natl. Acad. Sci. U.S.A. (2001) Jun 12; 98 (13). (ABSTRACT) (pdf)

Zhong, X.; Lizardi, P.M.; Huang, X.; Bray-Ward, P.; Ward, D.C. Visualization of Oligonucleotide Probes and Point Mutations in Interphase Nuclei and DNA Fibers Using Rolling Circle DNA Amplification. Proc. Natl. Acad. Sci. U.S.A. (2001) 98:3940-3945. (ABSTRACT) (pdf)

Ozcan,T., Burki, N., Parkash, V., Huang, X., Pejovic, T., Mahoney, M.J., Mari, G. and Ward, D.C. Cytogenetical Diagnosis in Paraffin-embedded Fetoplacental Tissue Using Comparative Genomic Hybridization. Prenatal Diagnosis. (2000) 20 (1): 41-44. (ABSTRACT) (pdf)

Lizardi, P.M.; Huang, X.; Zhu, Z.; Bray-Ward, P.; Thomas, D.C.; Ward, D.C. Mutation detection and single molecule counting using isothermal rolling circle amplification. Nature Genetics (1998) 19: 225-232. (ABSTRACT) (pdf)

Huang, X.; Boxer, S.G. Discovery of New Ligand Binding Pathways in Myoglobin by Random Mutagenesis. Nature Structural Biology (1994) 1 (4): 226-229. (ABSTRACT) (pdf)

Huang, X. Probing Ligand Binding Dynamics and Folding of Myoglobin Using a Random Library of Myoglobin Mutants with Single Amino Acid Mutations, Ph.D. Thesis Stanford University (1997).

Patents

Huang, X.; Roller, E. Genome Sequencing by Mostly Natural DNA Synthesis. Application No. (2009).

Huang, X. Methods and Systems for Direct Real-Time Sequencing of Single DNA Molecules. Application No. 61/121,809 (2008).

Barbee, K.D.; Huang, X. Methods and Devices for Biomolecular Arrays. Application No. 60/887,244 (2007).

Lizardi, P.M.; Huang, X. Single Molecule Analysis Target-Mediated Ligation of Bipartite Primers. Patent No. 6,316,229 (2001) (Full PDF)

Funding

Genome Sequencing by Natural DNA Synthesis on Amplified Clones (NIH/NHGRI R01 HG004804) (ABSTRACT)

Genome Sequencing by Ligation Using Nano-Arrays of Single DNA Molecules (NIH/NHGRI R21 R21HG04130) (ABSTRACT)

Career Award (NSF BES-0547193)

Massively Parallel Cloning and Sequencing of DNA (NIH/HNGRI R21 R21HG03587) (ABSTRACT)